产品名称 | 宿主菌 复制子 | 大肠 复制子 | 筛选标记 | 启动子 | 诱导系统 | Cas蛋白 | 荧光 蛋白 | |
pST001 | pAMβ1 | Ori | Kan | PbacA | TetR | nCas9(D10A) | EGFP | |
pST002 | pAMβ1 | Ori | Kan | PbacA | TetR | nCas9(D10A) | EGFP | |
pST003 | pAMβ1 | Ori | Kan | PbacA | TetR | nCas9(D10A) | EGFP | |
pST004 | pE194ts | Ori | Erm, Crm/Cam | PbacA | ||||
pST005 | repB | R6k | ErmG, Crm/Cam | P1 | TetR | FnCas12a | sfGFP | |
pST006 | repB | R6k | ErmG, Crm/Cam | P1 | TetR | FnCas12a | sfGFP | |
pST007 | repB | R6k | ErmG, Crm/Cam | P1 | TetR | FnCas12a | sfGFP | |
pST008 | pAMβ1 | Ori | Erm, Kan | TetR | nCas9(D10A) | EGFP | ||
pST009 | pAMβ1 | Ori | Erm, Kan, Tet/TcR | xylose | nCas9(D10A) | |||
pST010 | repB | pBBR1 | ErmG, Amp | P43 | IPTG | SpCas9 | ||
pST011 | R6k | ErmG | araBAD | |||||
pST012 | AMA1 | Ori | Hyg | TEF，gpdA，trpC | ||||
pST013 | pVS1 oriV | Kan, SmR | CaMV 35S | SpCas9 | ||||
pST014 | CloDF13 ori | Crm/Cam | araBAD | SpCas9 | mcherry | |||
pST015 | pRO1600 oriV | Ori, pRO1600 oriV | Kan | mSarlet | ||||
pST016 | pRO1600 oriV | Ori, pRO1600 oriV | Kan | DsRed2 | ||||
pST017 | pRO1600 oriV | Ori, pRO1600 oriV | Amp | DsRed2 | ||||
pST018 | pRO1600 oriV | Ori, pRO1600 oriV | GmR | CBE(BE3) | ||||
pST019 | pRO1600 oriV | Ori, pRO1600 oriV | GmR | CBE(BE3) | ||||
pST020 | pRO1600 oriV | Ori, pRO1600 oriV | Amp | |||||
pST021 | oriV | oriV | Tet/TcR | SpCas9 | ||||
pST022 | pE194ts | Ori | Kan | Pman | SpCas9 | |||
pST023 | pAMβ1 | pSC101 ori-RepA101(Ts) | Kan | P23，Pldh | SpCas9 | |||
pST024 | pE194ts | Ori | Kan | P43 | araBAD | CBE(Plug-in BE3) | ||
pST025 | pWV01 | Ori | Erm, Kan | P23，Pldh | nCas9(D10A) | |||
pST026 | pWV01 | Ori | Erm, Kan | P23，Pldh | nCas9(D10A) | |||
pST027 | repA | Ori | ErmG, Crm/Cam | Pfdx | SpCas9 | |||
pST028 | repA | Ori | Crm/Cam | Psyn | ||||
pST029 | pRO1600 oriV | Ori | Kan | mcherry | ||||
pST030 | Ori | Amp | sfGFP | |||||
pST031 | pRO1600 oriV | Ori | Amp | |||||
pST032 | pE194ts | Ori | Kan | nCas9(D10A) | ||||
pST033 | pE194ts | Ori | Kan | |||||
pST034 | pRO1600 oriV | Ori | GmR | |||||
pST035 | Ori | Kan | ||||||
pST036 | pRO1600 oriV | Ori | GmR | |||||
pST037 | Ori | Amp | ||||||
pST038 | pRO1600 oriV | Ori | Amp | |||||
pST039 | pE194ts | Ori | Kan, Crm/Cam | |||||
pST040 | pAMβ1 | Ori | Erm, Amp | PbacA | EGFP | |||
pST041 | pAMβ1 | Ori | Erm, Kan | PbacA | TetR | nCas9(D10A) | EGFP | |
pST042 | pAMβ1 | Ori | Erm, Kan | PbacA | TetR | CBE(Plug-in BE3) | ||
pST043 | pAMβ1 | Ori | Erm, Kan | PbacA | TetR | AsCas12a | ||
pST044 | pAMβ1 | Ori | Erm, Kan | PbacA | TetR | CBE(BE3) | ||
pST045 | pAMβ1 | Ori | Erm, Kan | PbacA | TetR | |||
pST046 | pAMβ1 | Ori | Erm, Kan | PbacA | TetR | nCas9(D10A) | ||
pST047 | pAMβ1 | Ori | Erm, Kan | PbacA | TetR | CBE(TargetAID) | ||
pST048 | pAMβ1 | Ori | Erm, Kan | PbacA | TetR | nCas9(D10A) | ||
pST049 | pAMβ1 | Ori | Erm, Kan | PbacA | TetR | nCas9(D10A) | ||
pST050 | pAMβ1 | Ori | Erm, Kan | PbacA | TetR | nCas9(D10A) | ||
pST051 | pAMβ1 | Ori | Erm, Kan | PbacA | TetR | nCas9(D10A) | ||
pST052 | pAMβ1 | Ori | Erm, Kan | PbacA | TetR | nCas9(D10A) | EGFP, mcherry | |
pST053 | pAMβ1 | Ori | Erm, Kan | PbacA | TetR | nCas9(D10A) | EGFP, mcherry | |
pST054 | pAMβ1 | Ori | Erm, Kan | PbacA | TetR | FrCas9 | ||
pST055 | pAMβ1 | Ori | Erm, Kan | PbacA | TetR | SpCas9 | ||
pST056 | pAMβ1 | Ori | Erm, Kan | PbacA | TetR | SpCas9 | ||
pST057 | pWV01 | Ori | Erm, Kan | P23，Pldh | SpCas9 | sfGFP | ||
pST058 | pWV01 | Ori | Erm, Kan | P23，Pldh | nCas9(D10A) | EGFP | ||
pST059 | pWV01 | Ori | Erm, Kan | P23，Pldh | FrCas9 | EGFP | ||
pST060 | pWV01 | Ori | Erm, Kan | P23，Pldh | SpCas9 | EGFP | ||
pST061 | pWV01 | Ori | Erm, Amp, Crm/Cam | PbacA | TetR | nCas9(D10A) | EGFP | |
pST062 | pWV01 | Ori | Erm, Amp, Crm, Erm, Amp, Crm/Cam | PbacA | TetR | SpCas9 | EGFP | |
pST063 | pWV01 | Ori | Erm, Crm/Cam | PbacA，P32 | TetR | nCas9(D10A) | EGFP | |
pST064 | pWV01 | Ori | Erm, Crm/Cam | PbacA，P32 | TetR | nCas9(D10A) | EGFP | |
pST065 | pWV01 | Ori | Erm, Crm/Cam | TetR | nCas9(D10A) | EGFP | ||
pST066 | pWV01 | Ori | Erm, Crm/Cam | TetR | SpCas9 | EGFP | ||
pST067 | pWV01 | Ori | Crm/Cam | PbacA | nCas9(D10A) | sfGFP | ||
pST068 | pWV01 | Ori | Crm/Cam | PbacA | SpCas9 | sfGFP | ||
pST069 | pWV01 | Ori | Erm, Kan | PbacA | SpCas9 | EGFP | ||
pST070 | pWV01 | Ori | Erm, Kan | PbacA | SpCas9 | EGFP | ||
pST071 | pWV01 | Ori | Erm, Kan | PbacA | SpCas9 | sfGFP | ||
pST072 | pWV01 | Ori | Erm, Kan | Pldh | SpCas9 | |||
pST073 | pRO1600 oriV | Ori, pRO1600 oriV | GmR | trc | araBAD | CBE(Plug-in BE3) | ||
pST074 | pRO1600 oriV | Ori, pRO1600 oriV | GmR | trc | araBAD | CBE(Plug-in BE3) | ||
pST075 | pRO1600 oriV | Ori, pRO1600 oriV | GmR | trc | araBAD | CBE(Plug-in BE3) | ||
pST076 | pRO1600 oriV | Ori, pRO1600 oriV | GmR | trc | araBAD | CBE(Plug-in BE3) | ||
pST077 | pRO1600 oriV | Ori, pRO1600 oriV | GmR | trc | araBAD | CBE(Plug-in TargetAID) | ||
pST078 | pRO1600 oriV | Ori, pRO1600 oriV | GmR | trc | araBAD | CBE(Plug-in TargetAID) | ||
pST079 | pRO1600 oriV | Ori, pRO1600 oriV | GmR | trc | araBAD | CBE(Plug-in BE3) | sfGFP, mcherry | |
pST080 | pRO1600 oriV | Ori, pRO1600 oriV | GmR | trc | araBAD | CBE(Plug-in BE3) | sfGFP, mcherry | |
pST081 | pRO1600 oriV | Ori, pRO1600 oriV | GmR | trc | araBAD | CBE(Plug-in BE3) | sfGFP | |
pST082 | pRO1600 oriV | Ori, pRO1600 oriV | GmR | trc | araBAD | CBE(Plug-in TargetAID) | sfGFP, mcherry | |
pST083 | pRO1600 oriV | Ori, pRO1600 oriV | GmR | trc | araBAD | CBE(Plug-in TargetAID) | sfGFP, mcherry | |
pST084 | pRO1600 oriV | Ori, pRO1600 oriV | GmR | trc | araBAD | CBE(BE3) | ||
pST085 | pRO1600 oriV | Ori, pRO1600 oriV | GmR | trc | araBAD | CBE(BE3) | ||
pST086 | oriV | oriV | Tet/TcR | Pldh | araBAD | CBE(BE3) | ||
pST087 | oriV | oriV | Tet/TcR | Pldh | araBAD | CBE(BE3) | ||
pST088 | oriV | oriV | Tet/TcR | Pldh | araBAD | CBE(BE3) | ||
pST089 | pRO1600 oriV | Ori, pRO1600 oriV | GmR | trc | araBAD | CBE(TargetAID) | ||
pST090 | pRO1600 oriV | Ori, pRO1600 oriV | Amp | trc | araBAD | |||
pST091 | repB | R6k | ErmG, Amp | P1 | TetR | FnCas12a | sfGFP | |
pST092 | repB | R6k | ErmG, Amp, Kan | P1 | TetR | FnCas12a | sfGFP | |
pST093 | repB | R6k | ErmG, Amp | P1 | TetR | FnCas12a | sfGFP | |
pST094 | repB | R6k | ErmG, Amp | P1 | TetR | FnCas12a | sfGFP | |
pST095 | repB | R6k | ErmG, Amp | P1 | TetR | FnCas12a | sfGFP | |
pST096 | repB | Ori | Kan, ErmG | trc,PbacA | TetR | nCas9(D10A) | EGFP | |
pST097 | repB | Ori | Kan, ErmG | trc,PbacA | TetR | nCas9(D10A) | EGFP | |
pST098 | pAMβ1 | Ori | Erm | TetR | AsCas12a | |||
pST099 | pAMβ1 | Ori | Erm, Kan | TetR | CBE(TargetAID) | |||
pST100 | pAMβ1 | Ori | Erm, Kan | TetR | nCas9(D10A) |
pST101 | pAMβ1 | Ori | Erm, Kan | TetR | nCas9(D10A) | |||
pST102 | pAMβ1 | Ori | Erm, Kan | TetR | nCas9(D10A) | |||
pST103 | pAMβ1 | Ori | Erm, Kan | TetR | nCas9(D10A) | |||
pST104 | pAMβ1 | Ori | Erm, Kan | TetR | nCas9(D10A) | |||
pST105 | oriV | Tet/TcR | Pldh，PbacA | |||||
pST106 | oriV | Tet/TcR | Pldh，PbacA | |||||
pST107 | repB | Ori | ErmG, Crm/Cam | T5，J23119 | IPTG | ChCas12f1 | ||
pST108 | repB | Ori | ErmG, Crm/Cam | T5，J23119 | IPTG | ChCas12f1 | ||
pST109 | repB | R6k | ErmG, Amp | P1 | TetR, araBAD | FnCas12a | sfGFP | |
pST110 | repB | Ori | ErmG, Kan | Pldh，PbacA | TetR, araBAD | nCas9(D10A) | EGFP | |
pST111 | oriV | Tet/TcR, Hyg | Pldh，PbacA | araBAD | nCas9(D10A) | |||
pST112 | oriV | Tet/TcR, Hyg | Pldh，PbacA | araBAD | SpCas9 | |||
pST113 | oriV | Tet/TcR, Hyg | Pldh，PbacA | araBAD | ||||
pST114 | oriV | Tet/TcR, Hyg, ErmG | Pldh，PbacA | araBAD | ||||
pST115 | pAMβ1 | Ori | Erm, Kan | TetR | CBE(BE3) | |||
pST116 | RepEDG-Kan-Erm-TetR-dead BE3-oriT-GOBsgRNA4 | pAMβ1 | Ori | Erm, Kan | TetR | CBE(BE3) | ||
pST117 | RepEDG-Kan-Erm-TetR-dead BE3-oriT-GOBsgRNA5 | pAMβ1 | Ori | Erm, Kan | TetR | CBE(BE3) | ||
pST118 | RepEDG-Kan-Erm-TetR-dSpCas9 -evoCDA1-oriT-GOBsgRNA2 | pAMβ1 | Ori | Erm, Kan | TetR | CBE(TargetAID) | ||
pST119 | RepEDG-Kan-Erm-TetR-dSpCas9 -evoCDA1-oriT-GOBsgRNA3 | pAMβ1 | Ori | Erm, Kan | TetR | CBE(TargetAID) | ||
pST120 | RepEDG-Kan-Erm-TetR-GoCas12m -APOBEC1-oriT-GOBsgRNA1 | pAMβ1 | Ori | Erm, Kan | TetR | GoCas12m-BE3 | ||
pST121 | RepEDG-Kan-Erm-TetR-GoCas12m -evoCDA1-oriT-GOBsgRNA1 | pAMβ1 | Ori | Erm, Kan | TetR | GoCas12m-evoCDA1 | ||
pST122 | RepEDG-Kan-Erm-TetR-MmCas12m -APOBEC1-oriT-GOBsgRNA1 | pAMβ1 | Ori | Erm, Kan | TetR | MmCas12m-BE3 | ||
pST123 | RepEDG-Kan-Erm-TetR-MmCas12m -evoCDA1-oriT-GOBsgRNA1 | pAMβ1 | Ori | Erm, Kan | TetR | MmCas12m-evoCDA1 | ||
pST124 | RepB-Kan-Erm-1PrtP | repB | Ori | ErmG, Kan | P32 | EGFP | ||
pST125 | RepB-Kan-Erm-1PrtP-EGFP | repB | Ori | ErmG, Kan | P32 | EGFP | ||
pST126 | RepB-Kan-Erm-2Cwh | repB | Ori | ErmG, Kan | P32 | EGFP | ||
pST127 | RepB-Kan-Erm-2Cwh-EGFP | repB | Ori | ErmG, Kan | P32 | EGFP | ||
pST128 | RepB-Kan-Erm-3Exp4 | repB | Ori | ErmG, Kan | P32 | EGFP | ||
pST129 | RepB-Kan-Erm-3Exp4-EGFP | repB | Ori | ErmG, Kan | P32 | EGFP | ||
pST130 | RepB-Kan-Erm-4SlpA | repB | Ori | ErmG, Kan | P32 | EGFP | ||
pST131 | RepB-Kan-Erm-4SlpA-EGFP | repB | Ori | ErmG, Kan | P32 | EGFP | ||
pST132 | RepB-Kan-Erm-5USP45 | repB | Ori | ErmG, Kan | P32 | EGFP | ||
pST133 | RepB-Kan-Erm-5USP45-EGFP | repB | Ori | ErmG, Kan | P32 | EGFP | ||
pST134 | RepEDG-Kan-Erm-1PrtP | pAMβ1 | Ori | Erm, Kan | P32 | EGFP | ||
pST135 | RepEDG-Kan-Erm-2Cwh | pAMβ1 | Ori | Erm, Kan | P32 | EGFP | ||
pST136 | RepEDG-Kan-Erm-3Exp4 | pAMβ1 | Ori | Erm, Kan | P32 | EGFP | ||
pST137 | RepEDG-Kan-Erm-4SlpA | pAMβ1 | Ori | Erm, Kan | P32 | EGFP | ||
pST138 | RepEDG-Kan-Erm-5USP45 | pAMβ1 | Ori | Erm, Kan | P32 | EGFP | ||
pST139 | RepEDG-Kan-Erm-TetR-D10A -EGFP-KI-BLF-sgRNA1 | pAMβ1 | Ori | Erm, Kan | P32 | TetR | nCas9(D10A) | EGFP |
pST140 | RepEDG-Kan-Erm-TetR-D10A -EGFP-KI-BLF-sgRNA2 | pAMβ1 | Ori | Erm, Kan | P32 | TetR | nCas9(D10A) | EGFP |
pST141 | RepEDG-TetR-EGFP-Kan-BLF-Erm | pAMβ1 | Ori | Erm, Kan | P32 | TetR | EGFP | |
pST142 | pAMβ1-Kan-Erm-TetR-D10A- fsrB-AtoT-sgRNA | pAMβ1 | Ori | Erm, Kan | TetR | nCas9(D10A) | EGFP | |
pST143 | pAMβ1-Kan-Erm-TetR-D10A- fsrB-AtoT-sgRNA2 | pAMβ1 | Ori | Erm, Kan | TetR | nCas9(D10A) | EGFP | |
pST144 | pAMβ1-Kan-Erm-Tet-xylose- D10A-fsrB-AtoT-sgRNA | pAMβ1 | Ori | Erm, Kan, Tet/TcR | xylose | nCas9(D10A) | EGFP | |
pST145 | pLCNICK-prgB-EGFP-prgC-ldhsgRNA | pAMβ1 | Ori | Amp | EGFP | |||
pST146 | pLCNICK-prgB-EGFP-prgC-1 | pAMβ1 | pSC101 ori-RepA101(Ts) | Erm, Kan | Pldh | EGFP | ||
pST147 | RepEDG-Amp-Erm-prgB- EGFP-prgC-prgUsgRNA | pAMβ1 | Ori | Amp, Erm | PbacA | EGFP | ||
pST148 | pLCNICK-prgB-EGFP-prgC -prgUsgRNA | pAMβ1 | Ori | Erm | EGFP | |||
pST149 | RepEDG-Kan-Erm-TetR-D10A-ldh-KI- mCherry-oriT-AW-ldhAsgRNA355 | pAMβ1 | Ori | Erm, Kan | PbacA | TetR | nCas9(D10A) | mcherry |
pST150 | pAMβ1-Kan-Erm-TetR-D10A-oriT -prgU-EGFP-sgRNA | pAMβ1 | Ori | Erm, Kan | PbacA | TetR | nCas9(D10A) | EGFP |
pST151 | pUC57-gbcas9 | Ori | Amp | gbCas9 | ||||
pST152 | pUC57-zpcas9 | Ori | Amp | zpCas9 | ||||
pST153 | pet28a-zpCas9-tracrRNA+DR | Ori | Kan | J23119 | IPTG | zpCas9 | ||
pST154 | pet28a-FcCas9-tracrRNA+DR | Ori | Kan | J23119 | IPTG | |||
pST155 | pet28a-frcas9√，又名pet28a -FrCas9-PAM耗竭阳性对照 | Ori | Kan | J23119 | IPTG | FrCas9 | ||
pST156 | pet28a-gbCas9-tracrRNA+DR | Ori | Kan | J23119 | IPTG | gbCas9 | ||
pST157 | 6nt文库质粒图谱 | p15A ori | Amp | |||||
pST158 | pAMβ1-Kan-Erm-Tet-xylose -D10A-fsrB-AtoT-sgRNA2 | pAMβ1 | Ori | Erm, Kan, Tet/TcR | xylose | nCas9(D10A) | EGFP | |
pST159 | pUC19-crRNA1-ccdB | Ori | Amp | araBAD | ||||
pST160 | pUC19-crRNA1-ccdB-B1-dcas12a | Ori | Amp | araBAD | Beacon1 | |||
pST161 | pUC19-crRNA2-ccdB | Ori | Amp | araBAD | ||||
pST162 | pUC19-sgRNA1-ccdB-Cas9 | Ori | Amp | araBAD | SpCas9 | |||
pST163 | pUC19-sgRNA2-ccdB | Ori | Amp | araBAD | ||||
pST164 | pUC19-sgRNA2-ccdB-Cas9 | Ori | Amp | araBAD | SpCas9 | |||
pST165 | pUC19-sgRNA-ccdB | Ori | Amp | araBAD | ||||
pST166 | BAD-Cas12-ccdB | oriV | Tet/TcR | araBAD | Beacon1 | |||
pST167 | p11-LacY-sgRNA1-ccdB donor | Ori | Amp | araBAD | ||||
pST168 | p11-LacY-sgRNA2-ccdB donor | Ori | Amp | araBAD | ||||
pST169 | pUC19-2xcrRNA | Ori | Amp | |||||
pST170 | pB025 | repB | R6k | ErmG, Amp | P1 | TetR | FnCas12a | |
pST171 | pnCasPA-BEC | pRO1600 oriV | Ori, pRO1600 oriV | GmR | CBE(BE3) | |||
pST172 | pet28a-922，ok | Ori | Kan | J23119 | IPTG | 922Cas9 | ||
pST173 | 2-pet28a-ChCas12f1-DR | Ori | Kan | J23119 | IPTG | ChCas12f1 | ||
pST174 | 3-pet28a-ChCas12f1-DR | Ori | Kan | J23119 | IPTG | ChCas12f1 | ||
pST175 | 4-pet28a-ChCas12f1-tracr-DR | Ori | Kan | J23119 | IPTG | ChCas12f1 | ||
pST176 | 4th_26 | Ori | Kan | J23119 | IPTG | |||
pST177 | PET28-0507a22 | Ori | Kan | J23119 | IPTG | |||
pST178 | pet28a-9 | Ori | Kan | J23119 | IPTG |